# BARseq **Expanded name:** Barcoded Anatomy Resolved by Sequencing **Entity class:** Spatial transcriptomic and projection-mapping method **Collections:** [[collections/Neurotech|Neurotech]] · [[collections/Consciousness Continuity|Consciousness Continuity]] ## Definition **BARseq** uses nucleic-acid barcodes and in-situ sequencing to relate neuronal gene expression and spatial identity to long-range projection patterns at scale. ## Relationships - BARseq complements [[wiki/MERFISH|MERFISH]] and [[wiki/Patch-seq|Patch-seq]] by joining molecular classification to anatomical projection information. - [[wiki/PRISM (E11 Bio)|PRISM]] is an adjacent barcode-assisted optical tracing system: BARseq uses nucleic-acid barcodes and in-situ sequencing for projection and molecular mapping, while PRISM's reported platform uses combinatorial protein barcodes to support local reconstruction through gaps. - Its measurements contribute to reference atlases and [[wiki/Connectomics|connectomic]] interpretation, but they do not replace synapse-level wiring or dynamic neural-state capture. - The method is routed from [[articles/Technologies for Consciousness Mapping and Transfer|Technologies for Consciousness Mapping and Transfer]] and links back to [[collections/Neurotech|Neurotech]]. <!-- BEGIN HUMANIZED RELATIONSHIPS 2026-09-11 --> This entry's documented connections are expressed in its definition and related-work routes, with provenance retained in the source-linked material. <!-- END HUMANIZED RELATIONSHIPS 2026-09-11 --> ## Related Work in the Corpus <!-- BEGIN HUMANIZED CORPUS ROUTES 2026-09-11 --> - In [[articles/Technologies for Consciousness Mapping and Transfer|Technologies for Consciousness Mapping and Transfer]], **IV. The brain as [[wiki/Connectomic Reference Address Space|address space]]** provides the narrative context for **BARseq**: The connectome is also becoming typed. <!-- END HUMANIZED CORPUS ROUTES 2026-09-11 -->