# Calcium Imaging **Collection:** [[collections/Neurotech|Neurotech]] **Type:** Functional neural imaging method **Primary sources:** [[articles/2026 Annual Report on Brain-Computer Interfaces|2026 Annual Report on Brain-Computer Interfaces]]; [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] ## Definition **Calcium imaging** uses indicators whose fluorescence changes with intracellular calcium to infer activity in neurons and other cells. ## Relationships - [[wiki/MICrONS|MICrONS]] recorded responses from roughly 75,000 neurons before reconstructing the same tissue with [[wiki/Electron Microscopy|electron microscopy]]. - [[wiki/ZAPBench|ZAPBench]] uses whole-brain optical activity recording in larval zebrafish. - Calcium signals are temporally filtered proxies for electrical spiking, not direct voltage recordings. <!-- BEGIN NEUROTECH RELATIONSHIP GRAPH 2026-09-10 --> ### Master relationship graph patch — 2026-09-10 **Collection:** [[collections/Neurotech|Neurotech]] **Relationship source:** [[research/Neurotechnology Ecosystem Relationship Graph - 2026-09-10|Neurotechnology Ecosystem Relationship Graph — 2026-09-10]] **Related source articles:** [[articles/The Architecture of Continuity and Emerging Neuroinformatics Standards|The Architecture of Continuity and Emerging Neuroinformatics Standards]] · [[articles/2026 Annual Report on Brain-Computer Interfaces|2026 Annual Report on Brain-Computer Interfaces]] · [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] #### Incoming typed edges - **Edge 347 — [[wiki/MICrONS|MICrONS]] `integrates` → this entry** — **CORPUS**; evidence `CORPUS_ATLAS`, `MICRONS_ALLEN`. Function-structure registration. #### Epistemic boundary `VERIFIED`, `VERIFIED_SECONDARY`, `CORPUS`, `STRONG_TECHNICAL_LINEAGE`, `STRONG_TECHNICAL_CONVERGENCE`, `ANALYTIC`, and `UNRESOLVED` are retained as distinct relation states. An edge does not imply ownership, control, funding, licensing, technology transfer, or organizational identity unless its predicate and evidence state that relationship explicitly. <!-- END NEUROTECH RELATIONSHIP GRAPH 2026-09-10 -->