# FISSEQ **Expanded name:** Fluorescent in situ sequencing **Entity class:** Spatial molecular sequencing method **Collection:** [[collections/Neurotech|Neurotech]] **Canonical source articles:** [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] · [[articles/Technologies for Consciousness Mapping and Transfer|Technologies for Consciousness Mapping and Transfer]] ## Definition **FISSEQ** sequences RNA while retaining spatial coordinates inside fixed cells and tissues. It joins molecular identity to location rather than discarding tissue context during bulk extraction. FISSEQ is part of the spatial-molecular-measurement lineage surrounding [[wiki/George Church|George Church]] and [[wiki/Kathleen G. C. Leeper|Kathleen G. C. Leeper]]. It is relevant to PRISM's strategy of preserving identity at spatial coordinates, but PRISM uses cell-filling protein bits and iterative antibody detection rather than in-situ nucleic-acid sequencing. ## Relationships - **Lead-inventor lineage:** [[wiki/George Church|George Church]]. - **Laboratory-lineage bridge:** [[wiki/Kathleen G. C. Leeper|Kathleen G. C. Leeper]]. - **Technical ancestry and comparator:** [[wiki/PRISM (E11 Bio)|PRISM (E11 Bio)]]. - **Collection route:** [[collections/Neurotech|Neurotech]]. ## Sources - [Wyss Institute — FISSEQ](https://wyss.harvard.edu/technology/fluorescent-in-situ-sequencing-fisseq/) - [Nature Protocols/PubMed — FISSEQ of RNA in intact cells and tissues](https://pubmed.ncbi.nlm.nih.gov/25675209/)