# MAPseq **Expanded name:** Multiplexed Analysis of Projections by Sequencing **Entity class:** Nucleic-acid-barcode projection-mapping method **Collection:** [[collections/Neurotech|Neurotech]] **Canonical source articles:** [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] · [[articles/Technologies for Consciousness Mapping and Transfer|Technologies for Consciousness Mapping and Transfer]] ## Definition **MAPseq** labels neurons with random RNA barcodes, transports barcode RNA into axon terminals, and sequences target regions to recover many single-neuron projection profiles in parallel. It converts projection mapping into a sequencing problem with a very large identity space. MAPseq is a technical ancestor and comparator for PRISM, not an equivalent implementation. Its sequencing readout can map projections at high throughput, but it does not by itself provide a continuously cell-filling morphology signal along the imaged neurite for segmentation. ## Relationships - **Method lineage:** nucleic-acid barcoding and high-throughput sequencing. - **Extended by:** [[wiki/BARseq|BARseq]], which preserves spatial soma information through in-situ sequencing. - **Comparator and ancestry:** [[wiki/PRISM (E11 Bio)|PRISM (E11 Bio)]]. - **Collection route:** [[collections/Neurotech|Neurotech]]. ## Sources - [Neuron/PMC — High-throughput mapping of single neuron projections by sequencing of barcoded RNA](https://pmc.ncbi.nlm.nih.gov/articles/PMC6640135/)