# Two-Photon Calcium Imaging **Entity class:** Concept or analytic term **Collection:** [[collections/Neurotech|Neurotech]] **Type:** Functional imaging method **Primary sources:** [[articles/2026 Annual Report on Brain-Computer Interfaces|2026 Annual Report on Brain-Computer Interfaces]]; [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] ## Relationships - It is a depth-resolved optical form of [[wiki/Calcium Imaging|calcium imaging]]. - [[wiki/Baylor College of Medicine|Baylor College of Medicine]] used it to record mouse visual-cortex responses for [[wiki/MICrONS|MICrONS]]. - The functional record was later co-registered with [[wiki/Electron Microscopy|electron-microscopy]] structure. <!-- BEGIN HUMANIZED RELATIONSHIPS 2026-09-11 --> This entry's documented connections are expressed in its definition and related-work routes, with provenance retained in the source-linked material. <!-- END HUMANIZED RELATIONSHIPS 2026-09-11 --> ## Related Work in the Corpus <!-- BEGIN HUMANIZED CORPUS ROUTES 2026-09-11 --> - In [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]], **V.1 — The Functional Imaging Stack** provides the narrative context for **Two-Photon Calcium Imaging**: Two-photon calcium imaging is the dominant cellular-resolution technique for monitoring large populations of neurons in living tissue. - In [[articles/2026 Annual Report on Brain-Computer Interfaces|2026 Annual Report on Brain-Computer Interfaces]], **MICrONS: Function-Structure Registration at Mammalian Scale** provides the narrative context for **Two-Photon Calcium Imaging**: The consortium of 150+ scientists across 22 institutions was led by the Allen Institute for Brain Science (Senior Investigator Dr. <!-- END HUMANIZED CORPUS ROUTES 2026-09-11 -->