# Two-Photon Calcium Imaging **Collection:** [[collections/Neurotech|Neurotech]] **Type:** Functional imaging method **Primary sources:** [[articles/2026 Annual Report on Brain-Computer Interfaces|2026 Annual Report on Brain-Computer Interfaces]]; [[articles/The Organic-Synthetic Brain Atlas|The Organic-Synthetic Brain Atlas]] ## Relationships - It is a depth-resolved optical form of [[wiki/Calcium Imaging|calcium imaging]]. - [[wiki/Baylor College of Medicine|Baylor College of Medicine]] used it to record mouse visual-cortex responses for [[wiki/MICrONS|MICrONS]]. - The functional record was later co-registered with [[wiki/Electron Microscopy|electron-microscopy]] structure.